Coding

Part:BBa_K1194004:Design

Designed by: Aman Kumar   Group: iGEM13_IIT_Madras   (2013-09-26)


pLuxR --> ompF --> Gb3 mimic


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The nine amino acid sequence had to be codon optimized for the optimal expression in E. coli.


Source

OmpF is from Escherichia coli. Gb3 mimic is a synthetic peptide taken from peptide library. pLuxR is taken from the parts registry.

References